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    Chiara SCHIRALDI

    Insegnamento di INDUSTRIAL BIOCHEMISTRY AND BIOTECHNOLOGY

    Corso di laurea magistrale in MOLECULAR BIOTECHNOLOGY

    SSD: BIO/10

    CFU: 6,00

    ORE PER UNITÀ DIDATTICA: 48,00

    Periodo di Erogazione: Primo Semestre

    Italiano

    Lingua di insegnamento

    INGLESE

    English

    Teaching language

    English

    Contents

    Introduction to industrial biochemistry, and biotechnological processes. The course is aiming at improving knowledge of biochemical and biotechnological updated techniques to obtain bioproducts of applicative interests. An in depth presentation of purification procedures with specific regards to pharmaceutical biotechnological products will be prented. Case studies on class of molecules will be introduced with a specific applicative focus (i.e. patent literature, updated established bioprocesses)

    Textbook and course materials

    Industrial Enzymology, Godfrey T. and West S.; Industrial microbiology:an introduction Waites M.J., Morgan N., Rockey JS, Higton G..

    Course objectives

    The course aims to provide advanced concepts on cells and biocatalyst application for industrial production. The students will acquired improved knowledge on downstream processes towards purification of bioactive molecules. Successively case studies will be presented and discussed.

    Prerequisites

    Students should have accomplished courses of biochemistry, fermentation biochemistry and biotechnology.
    Basic knowledge of separation processes, and principles of biochemical engineering may be useful as well

    Teaching methods

    Lectures using power point presentations.
    Practicals also with laboratory visit and one day experimental work.

    Evaluation methods

    Written report of the lab practice experience, eventually presenting the team work with a power point presentation

    Oral exam on the whole program

    Other information

    During the course specifically selected materials will be shared with the students. Spcifically, course notes, scientific article reprints, patents to be obtained from the Espacenet, or USpatent.gov web sites.

    Course Syllabus

    Cell programming for industrial production. Cells factories of proteins and other metabolites of application interest. Proteins from mesophilic and extremophilous microorganisms. Production of proteins from genetically modified organisms. Purification strategies; extracellular and intracellular enzymes; cell lysis and extraction. Inclusion bodies. Clarification of cell lysates; methods of pre-purification and purification. Scale-up of the extraction and purification process. Technical and economic implications of the purification strategy. Enzymes and proteins for industrial applications. Immobilized enzymes, immobilization techniques, specific bioprocesses and bioprocesses. Enzymes of interest in the transformation of carbohydrates: amylase, glucosidase, glucose isomerase. Enzymes degrading cellulose, hemicellulose, lignin and pectin. Lipases and their applications. Biotechnological production of short chain fatty acids and their industrial applications, description of current processes and current patent applications: production of citric acid and lactic acid. Stabilizing agents from natural sources and their potential biotechnological and biomedical applications: trehalose, ectoine and mannosil-glycerate in comparison. Bioprocesses for bacteriocins production from lactic bacteria. Notes on the biotechnological production of amino acids. Production and characterization of polysaccharides of cosmeceutical, nutraceutical and pharmaceutical interest: hyaluronic acid, heparin and chondroitin. Notes on the production of monoclonal antibodies with fed-batch cultures of transformed mammalian cells. Purification train for monoclonal antibodies, diagnostic and pharmaceutical use. Metabolic engineering: methods, definitions, case studies.Good Manufacturing practices, a specific approach for biotechnological products.

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