mail unicampaniaunicampania webcerca

    Nicola ALESSIO

    Insegnamento di AFP MOLECULAR BIOLOGY

    Corso di laurea magistrale a ciclo unico in MEDICINA E CHIRURGIA

    SSD: BIO/11

    CFU: 1,00

    ORE PER UNITÀ DIDATTICA: 20,00

    Periodo di Erogazione: Primo Semestre

    Italiano

    Lingua di insegnamento

    INGLESE

    English

    Teaching language

    English

    Contents

    Advanced Biomolecular Technologies

    Textbook and course materials

    Molecular Biology Techniques
    A Classroom Laboratory Manual
    4th Edition

    Authors: Sue Carson, Heather B. Miller, Melissa C. Srougi, D. Scott Witherow

    Course objectives

    The student will acquire fundamental knowledge underlying molecular biology techniques.

    Prerequisites

    Having attended the course in molecular biology.

    Teaching methods

    Students will be divided into working groups, with each group assigned to witness the experimental procedures objcted in this course

    Evaluation methods

    Oral exam with skill assessment of the student with final judgement: Eligibility, NON Eligibility

    Other information

    None

    Course Syllabus

    Agarose Gel Electrophoresis.
    PCR General Principles and Applications.
    RT-PCR, Real-time PCR.
    Restriction Enzymes.
    Types of Restriction-Modification Systems.
    Cloning Basics: Main Types of Plasmids. Selective Markers. Viral Vectors. Chemical Transformation and Electroporation.

    facebook logoinstagram buttonyoutube logotype